| 质粒名称: | pSXneo 270(T2AG3) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12403 |
| 存储实验室: | Titia de Lange |
|
载体骨架基本信息 |
|
| 载体名称: | pSP73 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 270(T2AG3),1.6 kb TTAGGG repeat,139 |
| 物种来源: | -- |
| 插入大小: | 1600 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Bgl II (未破坏) |
| 3' 克隆位点: | Sal I（在克隆过程中被破坏） |
| 5' 测序引物 | Sp6 |
| 3' 测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | pSXneo 270(T2AG3) was a gift from Titia de Lange (Addgene plasmid # 12403 ; http://n2t.net/addgene:12403 ; RRID:Addgene_12403) |
| 参考文献部分: | Stringent sequence requirements for the formation of human telomeres. Hanish JP, Yanowitz JL, de Lange T. Proc Natl Acad Sci U S A. 1994 Sep 13. 91(19):8861-5. 10.1073/pnas.91.19.8861 PubMed 8090736 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pSXneo 270(T2AG3) 3747 bp ds-DNA circular SYN 05-OCT-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSXneo 270(T2AG3)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3747)
AUTHORS Hanish JP, Yanowitz JL, de Lange T
TITLE Stringent sequence requirements for the formation of human
telomeres.
JOURNAL Proc Natl Acad Sci U S A. 1994 Sep 13. 91(19):8861-5.
PUBMED 8090736
REFERENCE 2 (bases 1 to 3747)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 5, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3747
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..20
/label=T7
/note="T7 promoter, forward primer"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 71..119
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(126..929)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(237..256)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 847..866
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(1402..1420)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(1403..1420)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(1501..1520)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1620..1642
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1680..1698)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1766..1870
/gene="bla"
/label=AmpR promoter
CDS 1871..2731
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2089..2108)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2902..3490
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3391..3410
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3644..3661
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 taatacgact cactataggg agaccggcct cgacctgcag gcatgcaagc ttcagctgct
61 cgacggatcc gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccga
121 tcccctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
181 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
241 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
301 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
361 gtcacgacga gatcatcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
421 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
481 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
541 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
601 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
661 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
721 agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
781 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
841 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
901 aatccatctt gttcaatggc cgatcccata ttggctgcag ggtcgctcgg tgttcgaggc
961 cacacgcgtc accttaatat gcgaagtgga cctgggaccg cgccgccccg actgcatctg
1021 cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgctcgacat tgggtggaaa
1081 cattccaggc ctgggtggag aggctttttg cttcctcttg caaaaccaca ctgctcgaca
1141 ttgggtggaa acattccagg cctgggtgga gaggcttttt gcttcctctt gaaaaccaca
1201 ctgctcgact ctagaggatc cccgggtacc gagctcgaat tccgcggaat tccggttggg
1261 gttggggttg gggttggggt tggggttagg gttggggtta gggttagggt tagggttagg
1321 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tcggaattgc
1381 ggccgcatcg atagatctgg ttctatagtg tcacctaaat cgtatgtgta tgatacataa
1441 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt gcactctcag
1501 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
1561 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
1621 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
1681 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
1741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
1801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
1861 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
1921 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
1981 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
2041 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
2101 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
2161 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
2221 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
2281 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
2341 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
2401 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
2461 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
2521 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
2581 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
2641 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
2701 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
2761 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
2821 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
2881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
2941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
3001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
3061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
3121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
3181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
3241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
3301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
3361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
3421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
3481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
3541 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
3601 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
3661 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
3721 atgcaggtta acctggctta tcgaaat
//
